This course offers an overview of current massive parallel sequencing data (NGS) paying special attention to those produced by the Illumina sequencing technology and its main applications to Genomics Research. Additionally, provides a basic training in bioinformatics techniques for handling, managing and analyzing NGS data. It will follow the mapping-based approach for calling Single Nucleotide Variants (SNVs) recommended by GATK (Broad Institute). Additionally, we will do several exercises for handling, filtering and extracting information from VCFs and GVCFs. Finally, these data will allow the estimation of basic statistics in Population Genomics.