The 2017 edition of this course offers an overview of the current massive parallel sequencing data paying special attention to those produced by the Illumina sequencing technology and its main applications for Genomic Research. Additionally, provides basic training in bioinformatic techniques for handling, managing and analyzing NGS data (e.g., using available bioinformatic programs and unix commandline options). We will focus on the mapping-based approach for variant calling recommended by the Broad Institute, following the GATK Best Practices. Finally, we will do several excercises for filtering files containing Single Nucleotide Variants (SNVs) and calculating classical population genetics statistics.