This course offers an overview of the current massive parallel sequencing data paying special attention to those produced by the Illumina sequencing technology and its main applications to Genomics Research. Additionally, provides a basic training in bioinformatic techniques for handling, managing and analyzing NGS data (e.g., using available bioinformatic programs and high-level dynamic programming languages). Finally, focuses on the bioinformatic protocols for variant calling and some of the analyses carried out with Single Nucleotide Polymorphisms (SNPs) in Population Genomics studies.